Analysis Note

ControlWestern Blot::
NIH-3T3 (100% confluent) lysate
Immunocytochemistry:
Mouse 3T3-Src(Y527F) cells

Application

Anti-TKS5 (SH3 #1) Antibody detects level of TKS5 (SH3 #1) & has been published & validated for use in WB, IC & IP.

Western Blot Analysis: Antibody detected TKS5 in 20 µg of NIH-3T3 (100% confluent) lysate.

Research CategoryCell Structure

Research Sub CategoryCytoskeletal Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Tks5 (previously known as Fish), is a large scaffolding protein with one amino-terminal PX domain and five SH3 domains. It is cytoplasmic in normal fibroblasts. In Src-transformed cells, Tks5 localizes to podosomes. The coexpression of Tks5 and constitutively active Src in epithelial cancer cells resulted in the appearance of podosomes/invadopodia. Cells with reduced Tks5 expression are poorly invasive through matrigel. Tks5 is expressed and localized to podosomes/invadopodia in some invasive human cancer cell lines and tumor tissue, particularly breast cancers and melanomas. Thus, Tks5 appears to be required for podosome/invadopodia formation and for invasion of some cancer cells.

Immunogen

Epitope: 166-225 aa

Recombinant Protein, aa: 166-225. Homology: Human, Dog, Horse, Rat, Cow (100%, 60/60); Chicken (93%, 56/60)

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Format: Purified

Purified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.

Quality

Routinely evaluated by Western Blot on NIH-3T3 (100% confluent) lysate.

Specificity

Other homologies: Human, Canine, Equine, Rat, and Bovine (100% sequence homology), Chicken (93% sequence homology)

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.

Target description

130-150 kDa Uncharacterized bands(s) may be observed in some cell lysates.